Prostate-specific antigen (PSA) was purified from
human seminal plasma by ion-exchange chromatography and gel filtration.
The purified antigen was dialyzed against d.H2O and
lyophilized.
Expressed in E. coli. DNA sequence encoding human
Vimentin (amino acid residues 1 – 466; Accession # X56134) was fused
with a His tag at the N-terminus.