Recombinant human Cbp / PAG

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	RL-004-C100	Lyophilized	0.1 mg		290 EUR

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Source

Expressed in E. coli. DNA sequence encoding intracellular domain of human Cbp/ PAG (amino acids 41- 432) was fused with a His tag at the N-terminus.

Description

Csk binding protein, also known as PAG (Phosphoprotein Associated with Glycosphingolipid-enriched microdomains) is a ubiquitously expressed transmembrane adaptor protein present in membrane rafts. Cbp/PAG has a pivotal role in the assembly of negative regulators in these membrane domains.
Cbp/PAG consists of a short extracellular domain (16 to 18 residues), a transmembrane domain (20 residues), and a large cytoplasmic domain (387 to 396 residues); it contains ten tyrosine residues, nine of which are potential substrates for Src kinases, and two proline-rich regions.

Database Links

AfCS Nature Molecule Page

Applications:

Loading control

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse Monoclonal to PAG / Cbp MEM-255 (IgG2a)

Mouse Monoclonal to PAG / Cbp PAG-C1 (IgG2b)

Rabbit Polyclonal to PAG / Cbp

Example Data

Fig. 1. SDS-PAGE

Fig.1. SDS-PAGE Electrophoresis of Cbp / PAG recombinant protein (cat. no. RL-004-C100). 12% separating gel, 6% stacking gel; reducing conditions.
Lane 1-5: Cbp / PAG recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomasie Blue.

Fig. 2. Western Blotting

Fig.2. Western Blotting analysis of Cbp / PAG recombinant protein (cat. no. RL-004-C100). Reducing conditions.
Lane 1-4: immunostaining with anti-Cbp rabbit polyclonal antibody (EXBIO cat. no. 11-409-C100; dilution 0.5 μg/ml). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of Cbp/PAG recombinant protein loaded on each lane: (1) 100 ng, (2) 50 ng, (3) 20 ng, (4) 10 ng.