Recombinant human TRAIL-R1 (DR4/ TNFRSF10A)/Fc Chimera

	Product No.	Form	Quantity	Stock	Price	Datasheet
	RL-001-C050	Lyophilized	0.05 mg		290 EUR

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Source

Produced in HEK 293 cells. The extracellular domain of human TRAIL-R1 (aa 1 - 238) is fused to the Fc portion of human IgG1.

Description

DR4 is a transmembrane protein, also called TRAIL receptor 1 (TRAIL-R1). The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

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Applications:

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Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

Unpublished.

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse Monoclonal to CD261 / TRAIL-R1 DR-4-02 (IgG1)

Example Data

Fig. 1. SDS-PAGE

Fig.1. SDS-PAGE Electrophoresis of TRAIL-R1 (DR4) / Fc recombinant protein (12% separating gel; reducing conditions).
Lane 1 to 3: TRAIL-R1 (DR4)/ Fc recombinant protein (2000 ng, 1000 ng, 500 ng per lane). Staining with Coomasie Blue.

Fig. 2. Western Blotting

Fig.2. Western Blotting of TRAIL-R1 (DR4) / Fc recombinant protein. Reducing conditions.
Lane 1 to 5: Immunostaining with TRAIL-R1 (DR4) specific antibody DR-4-02 (Cat. No. 10-403-C100; dilution 2 μg/ml ). Detection with Goat anti-mouse / HRP secondary antibody. Amount of TRAIL-R1 (DR4)/ Fc recombinant protein loaded on each lane: (1) 370 ng, (2) 150 ng, (3) 75 ng, (4) 37 ng, (5) 15 ng.