One-Step RT-PCR MasterMix

Spherotech, Inc.
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Exalpha Biologicals, Inc.
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EzWay™ One-Step RT-PCR MasterMix

Catalog Number	K0569600
Size	1ml
Type	PCR Kit
Datasheet

Description

EzWay™ One-Step RT-PCR MasterMix is designed for sensitive and specific amplification in one-tube reaction from total RNA transcripts.
EzWay™ One-Step RT-PCR MasterMix is a premixed buffer solution containing MMLV Reverse Transcriptase and Hot Taq DNA Polymerase, dNTPs, tracking & loading dye and stabilizers. The solution contains the proper concentration of MgCl2 and dNTPs for the better specificity and sensitivity and supplied as 2X concentrated type for convenience.
Simply add primers and RNA template diluted in DEPC-treated water into equal volume of EzWay™ One-Step RT-PCR MasterMix. Pipetting steps are minimized, reducing the possibility of errors and contamination.

Features

Synthesis of cDNA and sequentially PCR amplification from target gene in one-tube.
Specialized buffer composition for highly sensitive and specific RNA amplificatio
Simple set-up and minimal optimization required

Application

Rapid detection of target gene transcript from total RNA or purified mRNA

Packing

Component	Cat. No
2X EzWay™ One-Step RT-PCR MasterMix	K0569600
4X Magic Buffer	K0561031

RT-PCR of Beta-actin(275bp), c-Myc(215bp) and GAPD