Hot Universal qMasterMix

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN

GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

Tel + 32 16 58 90 45

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

Tel 01 43 25 01 50

Fax 01 43 25 01 60

GENTAUR Germany Marienbongard 20

52074 Aachen, Germany

Tel 0241 56 00 99 68 Fax 0241 56 00 47 88

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719

Danmark

+4569918806

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+358942419041

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+302111768494

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+35316526556

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+35220880274

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+3619980547

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+31208080893

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+4721031366

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+46852503438

Schweiz Züri
+41435006251

Northern America

Canada Montreal
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US New York
+17185132983

EzWay™ Hot Universal qMasterMix

Catalog Number	K0569010
Size	1ml
Type	PCR Kit
Datasheet

Description

2X EzWay™ Hot Universal qMasterMix is a premixed solution containing Hot Taq DNA Polymerase, dNTPs and the specialized reaction buffer for probe-based qPCR.
Hot Taq DNA Polymerase, a chemically modified form of Taq DNA Polymerase, sustains an inactive form at ambient temperature and activated by a 15 minutes at 95°C. Thus it provides high specificity in hot-start PCR. Also, the enzyme has 5'-nuclease activity required for some real time qPCR, e.g. TaqMan assay.
2X EzWay™ Hot Universal qMasterMix guarantees the highly specific and reproducible quantitative results. This solution does not contain ROX reference dye, which allows fluorescence normalization on cyclers, such as ABI7000, ABI7300, ABI7700, ABI7900HT, ABI7500, Stratagene Mx3000, Mx3005P, and MX4000.

Features

Real-time quantitative PCR of DNA and cDNA targets using fluorescent probe
High PCR specificity
High sensitivity
High reproducibility

Application

Real-Time quantification of DNA and cDNA targets
Gene expression profiling
Microbial & Viral pathogen detection

Packing

Component	Cat. No
2X EzWay™ Hot Universal qMasterMix	K0569010
4X Magic Buffer	K0561031

Human beta-Globin quantification by TaqMan with Hot Universal qMasterMix