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OUR SUPPLIERS
KOMABIOTECH
301,
Gayang Technotown, #1487
Gayang 3 dong,
Gangseo-gu
Seoul 157-793, KOREA
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
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EY
Laboratories, Inc. Headquarters
107 N.
Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
Sacace Biotechnologies S.r.l.
Via Scalabrini,
44
22100 Como Italy
GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
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SIRET 48423788800017
Rue Lagrange, 9
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France
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Marienbongard 20
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Germany
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Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
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23015 Milano, Italy
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0032 (0)16 41 44 07 |
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EzWay Direct ApoE Genotyping Kit
| Catalog Number |
K0568500 |
| Size |
50T |
| Type |
Kit |
| Datasheet |
 |
- ApoE genotyping directly from blood
- One-step Multiplex PCR system with PrimerMix
- ApoE primer mixture for E2 (Cys112/Cys158), E3
(Cys112/Arg158) and E4 (Arg112/Arg158)
- Fast and simple method for genotyping without
DNA purification
- Minimize the risk of DNA loss or contamination
- Optimized MasterMix type containing Taq enzyme,
dNTP, Direct PCR Buffer, MgCl2, Red dye and
additives
- Useful detection of high cholesterol levels,
cardiovascular disease or Alzheimer's disease
| 2X EzWay™ Direct Hot Taq PCR MasterMix |
625ul |
EzWay™ Direct PCR Buffer, Taq DNA
Polymerase, dNTP, MgCl2, Red dye and additive |
| 2.5X ApoE PrimerMix |
500ul |
Primer mixture for ApoE genotyping |
| DW |
500ul |
PCR grade DW |
| Size Marker |
50ul |
100bp DNA ladder |
- Determine the total number of samples including
negative control and prepare PCR tubes.
- Thaw 2X EzWay Direct Hot Taq PCR MasterMix and
2.5X ApoE PrimerMix, and vortex.
- Spin the tubes briefly in a microcentrifuge.
- Dispense 12.5ul EzWay Direct Hot Taq PCR
MasterMix, 10ul ApoE PrimerMix, 1.5ul distilled
water and 1ul blood sample into PCR tubes, and then
mix thoroughly. For negative control, use 1ul
distilled water instead of blood.
- Place the PCR tubes in a thermal cycler and
perform the PCR reaction immediately.
- Separate PCR products and size marker on 2%
agarose gel electrophoresis in 1X or 0.5X TAE buffer
and stain.
Blood was amplified directly using EzWay Direct ApoE
Genotyping Kit. One-step Multiplex PCR enables to
get the result just in 3 hours.
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