Multiplex PCR MasterMix

Spherotech, Inc.
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Exalpha Biologicals, Inc.
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EzWay Multiplex PCR MasterMix (2x)

Catalog Number	K0567810
Size	1ml
Type	PCR Kit
Datasheet

Description

2X EzWay™ Multiplex PCR MasterMix is a premixed solution containing the optimized amount of Hot Taq DNA Polymerase, Multiplex PCR Buffer, MgCl2, dNTPs and a dye.
Room-temperature reaction setup using 2X EzWay™ Multiplex PCR MasterMix is fast and easy. Simply add 1 volume of primers and template DNA diluted in the distilled water into equal volume of 2X EzWay™ Multiplex PCR MasterMix.
Pipetting steps are minimized, reducing the possibility of errors and contamination.
The combination of high specificity and easy handling makes the 2X EzWay™ Multiplex PCR MasterMix ideal for use with complex genomic or cDNA templates, multiple primer pairs, templates isolated from difficult sources, genetic screening in which large numbers of samples are amplified, and laboratory PCR automation.

Features

Multiplex PCR
High PCR specificity
Reduced non-specific amplification
Simple reaction setup at room temperature
Minimal optimization required
High reproducibility

Application

Multiplex PCR, PCR-based DNA fingerprinting (VNTR, STR, and RAPD)etc
Highly Specific PCR
Low Copy Number Target PCR. (e.g. Viral Detection in Blood)
RT-PCR of rare transcripts
Differential Display
Degenerate PCR

Packing

Component	Cat. No
2X EzWay™ Multiplex PCR MasterMix	K0567810
4X Magic Buffer	K0561031

Fig 1. Multiplex amplification of 12 targets (81~1,086 bp) was carried out for 35 cycles using EzWayTM Multiplex PCR MasterMix.

Fig 2. Amplification of 3 target genes having high GC content was performed with various amount of Magic Buffer (0~25%).