Hot Taq PCR

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

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Hot Taq DNA Polymerase Kit

Catalog Number	K0567100
Size	250U
Type	PCR Kit
Datasheet

Description

Hot Taq DNA Polymerase, a chemically modified form of Taq DNA Polymerase, is supplied in an inactive state that has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer–dimers formed at low temperatures during PCR setup and the initial PCR cycle. This makes Hot Taq DNA Polymerase ideal for quantitative real-time PCR. Hot Taq DNA Polymerase is activated by 15-minute incubation at 95°C which can be incorporated into any existing thermal-cycler program. Every lot of Hot Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified.

Features

High specificity
Reduced non-specific amplification
High reproducibility
Extension rate: 2-4 kb/min at 72°C
Half-life: 10 min at 97°C, 60 min at 94°C
Amplification efficiency: ≥105 fold
5'→3' exonuclease activity
Extra A addition
Contaminating nucleases: No
Contaminating RNases: No
Contaminating proteases: No
Self-priming activity: No

Applications

Highly Specific PCR
Low Copy Number Target PCR.(e.g. Viral Detection in Blood)
RT-PCR of rare transcript
Real-time PCR
Differential Display
Multiplex PCR, PCR-based DNA fingerprinting (VNTR, STR, and RAPD)etc
Degenerate PCR

Packing

Component	Cat. No
Hot Taq DNA Polymerase	K0567100
10X Hot Taq PCR Buffer	K0567111
dNTP Mix (2.5mM each)	K1756011
10X Loading Buffer	K0561051
4X Magic Buffer	K0561031