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HybridoMax
Hybridoma Growth Supplement
As you may
have heard is the FC-210001 ORIGEN Hybridoma Cloning factor no longer
available.
GENTAUR is
pleased to inform you we have a replacement for the FC210001 ORIGEN
Hybridoma Cloning factor.
We have an
alternative product which enables you to easily prepare your own
Serum-Free Hybridoma Growth Medium.
The
HybridoMax Hybridoma Growth Supplement
replaces feeder layers or other conditioned media and growth factors
used in hybridoma production. HybridoMax increases the yield of
hybridomas surviving HAT selection, the cloning efficiency of B-cell
hybridomas, and the number of antibody producing colonies.
The
HybridoMax Hybridoma Growth Supplement is ready to use. Prepare the
serum free Hybridoma growth medium by dissolving 1 bottle of HybridoMax
(11 ml) into 1 Liter of D-MEM F-12 (Dulbecco's Modified Eagle's Medium
F-12).
The
prepared Serum-Free Hybridoma growth medium is a chemically defined
culture medium supporting the serum-free growth of various hybridomas
and 293 cells. The compounds of Hybridoma growth medium include water
for injection, a modified DMEM/F12 base, HEPES buffer, known amounts of
insulin, transferin, testosterone, sodium selenite, ethanolamine, a
variety of saturated and unsaturated fatty acids, and stabilizing
proteins. HybridoMax contains no IL-6 and no bovine serum albumine or
other undefined protein mixtures.
HybridoMax
Hybridoma Growth Supplement, 11 ml bottles (for 1 Liter of Hybridoma
Growth Medium)
1 bottle
of HybridoMax for 57 Euro. (19-HYB-01)
5 bottles
of HybridoMax for 270 Euro. (19-HYB-05)
10 bottles
of HybridoMax for 510 Euro. (19-HYB-10)
You can
find the datasheet and the protocol here:
Datasheet.pdf
Protocol.pdf
This protocol can be followed when using the TX-HYB supplemented
Hybridoma Growth Medium in combination with a 1000ml cell Bioreactor.
We
recommend to use the LM-D1223/500 DMEM – Ham’s F12 with L-Glutamine with
15mM HEPES together with the HybridoMax supplement to support optimal
hybridoma development and achieve an optimal cell density. These are
sold in bottles of 500 ml.
5 bottle
of LM-D1223/500 for 59 Euro
10 bottles
of LM-D1223/500 for 99 Euro.
20 bottles
of LM-D1223/500 for 169 Euro. |
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