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EzWay Annexin V-FITC Apoptosis Detection Kit
Catalog Number K29100
Size 100T
Type Kit
Datasheet
 
Features
  • Utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis
  • Annexin V FITC binds to the membranes of apoptotic cells
  • Detection by flow cytometry or fluorescence microscopy
 
Reading of Result
  • Non-apoptotic cells: Annexin-V negative and PI negative
  • Non-apoptotic cells: Annexin-V negative and PI negative
  • Necrotic cells or late apoptotic cells: Annexin-V positive and PI positive
 
Kit Contents
  • Annexin V-FITC
  • 5X Binding buffer
  • 5X Binding buffer
 
How to Use
  1. Cell preparation and rinsing cells with Binding Buffer
  2. Add Annexin V-FITC
  3. Add Propidium Iodide
  4. Incubate at 4℃ in the dark.
  5. Detection
 
Staining of healthy and apoptotic Jurkat cells using flow cytometry
Jurkat cells were incubated with 0.5ug/ml actinomycin D for 20 hours. Untreated cells are shown in the left panel, apoptotic induced cells are shown in the right panel.