Threshold Determination

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN

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Flow Cytometry Particles For Threshold Determination

The SPHERO™ Blank Calibration Particles are designed for the detmination of the flow cytometer's fluorescence threshold. It is important to determine if the autofluorescence level of a sample is above the fluorescence threshold of the instrument. To measure the fluorescence threshold of the instrument run the Blank Calibration Particles at the same instrument settings and determine if they are less fluorescent than the sample. The fluorescence threshold of the instrument can be quantitatively determined by calibrating the instrument using the same instrument settings with calibrated standards such as the Rainbow or Ultra Rainbow Calibration Particles.

SPHERO™ Blank Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
BCP-10-5	Blank Calibration Particles	10⁷/mL	1.0-1.4 µm	5 mL	$110.00
BCP-20-5	Blank Calibration Particles	10⁷/mL	1.8-2.2 µm	5 mL	$110.00
BCP-30-5	Blank Calibration Particles	10⁷/mL	3.0-3.4 µm	5 mL	$110.00
BCP-35-5	Blank Calibration Particles	10⁷/mL	3.5-4.0 µm	5 mL	$110.00
BCP-50-5	Blank Calibration Particles	10⁷/mL	5.0-5.9 µm	5 mL	$110.00
BCP-60-5	Blank Calibration Particles	10⁷/mL	6.0-6.4 µm	5 mL	$110.00
BCP-70-5	Blank Calibration Particles	10⁷/mL	6.5-8.0 µm	5 mL	$110.00
BCP-100-5	Blank Calibration Particles	5x10⁶/mL	8.0-12.9 µm	5 mL	$125.00
BCP-150-5	Blank Calibration Particles	5x10⁶/mL	13.0-17.9 µm	5 mL	$125.00
BCP-200-5	Blank Calibration Particles	0.5x10⁶/mL	18.0-24.9 µm	5 mL	$125.00
BCP-300-5	Blank Calibration Particles	0.5x10⁶/mL	25.0-35.0 µm	5 mL	$125.00