Calibration

Flow Cytometry Calibration Particles

The SPHERO™ Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly.

SPHERO™ Rainbow Calibration Particles - Contains a mixture of fluorophores that enable the Rainbow Calibration Particles to be excited at any wavelength from 365 nm to 650 nm - Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels in the flow cytometer with the same set of particles - Consists of set of several similar size particles with different fluorescence intensities in a convenient dropper bottle - Can be stored in the freezer and reused later since they can withstand repeated freezing and thawing
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RCP-20-5	Rainbow Calibration Particles, 4 peaks	10⁷/mL	1.8-2.2 µm	5 mL	$220.00
RCP-30-5 (6 peaks)	Rainbow Calibration Particles, 6 peaks	10⁷/mL	3.0-3.4 µm	5 mL	$240.00
RCP-30-5A (8 peaks)	Rainbow Calibration Particles, 8 peaks	10⁷/mL	3.0-3.4 µm	5 mL	$240.00
RCP-32-5	Rainbow Calibration Particles, 6 peaks	10⁷/mL	3.2 (+/- 0.1) µm	5 mL	$240.00
RCP-35-5	Rainbow Calibration Particles, 4 peaks	10⁷/mL	3.5-4.0 µm	5 mL	$240.00
RCP-60-5	Rainbow Calibration Particles, 6 peaks	10⁷/mL	6.0-6.4 µm	5 mL	$240.00
RCP-30-5A-2	Rainbow Calibration Particles, 2nd peak from the 8 peak set	10⁷/mL	3.0-3.4 µm	5 mL	$240.00

Downloadable NOTE

Rainbow Calibration Particles
Other Rainbow Calibration Particles individual peaks are available upon request. Please contact customer service.

SPHERO™ Ultra Rainbow Calibration Particles - Designed for routine calibration of the flow cytometer in all channels - Contain a mixture of fluorophores that enable the optimization of linearity, sensitivity, and resolution of the flow cytometer - Especially useful in the Far Red and Violet channels
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
URCP-38-2K	Ultra Rainbow Calibration Kit, 6 peaks (Blank, 2x10⁶/mL), (Fluorescent Particles, 10⁷/mL)	10⁷/mL after mixing	3.5-3.9 µm	1x3 mL 1x2 mL	$230.00
URCP-50-2K	Ultra Rainbow Calibration Kit, 6 peaks (Blank 2x10⁶/mL), (Fluorescent Particles 10⁷/mL)	10⁷/mL after mixing	5.0-5.9 µm	1x3 mL 1x2 mL	$235.00

Downloadable NOTE

Ultra Rainbow Calibration Particles

SPHERO™ Spectral Matching Calibration Particle Kits - Surface labeled polystyrene particles with commonly used fluorophores - Used to determine the linearity of the logarithmic amps and sensitivity of flow cytometers in specific channels - Supplied as individual products for use in specific channels of flow cytometers
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
ECFP-F1-5K	FITC Easy Calibration Kit, 5 peaks	2x10⁶/mL	3.0-3.4 µm	5x1 mL	$235.00
ECFP-F2-5K	PE Easy Calibration Kit, 5 peaks	2x10⁶/mL	3.0-3.4 µm	5x1 mL	$235.00
ECFP-F4-5K	PE-Cy5 Easy Calibration Kit, 5 peaks	2x10⁶/mL	3.0-3.4 µm	5x1 mL	$235.00

SPHERO™ Rainbow Linear Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RLP-30-5	Rainbow Linear Calibration Particles (For use in Linear Scale)	10⁷/mL	3.0-3.4 µm	5 mL	$240.00

SPHERO™ ACP Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
ACP-30-5K	Allophycocyanin Calibration Particles, 6 peaks	10⁷/mL	3.0-3.4 µm	2x5 mL	$230.00

SPHERO™ Yellow Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
YCP-70-5	Yellow Calibration Particles	10⁷/mL	6.0-8.0 µm	5 mL	$250.00